Molecular Basis of Enrofloxacin Translocation through OmpF, an Outer Membrane Channel of Escherichia coli - When Binding Does Not Imply Translocation
authors Mahendran, KR; Hajjar, E; Mach, T; Lovelle, M; Kumar, A; Sousa, I; Spiga, E; Weingart, H; Gameiro, P; Winterhalter, M; Ceccarelli, M
journal JOURNAL OF PHYSICAL CHEMISTRY B
keywords SITE-DIRECTED MUTAGENESIS; BETA-LACTAM ANTIBIOTICS; SINGLE-CHANNEL; FLUOROQUINOLONE RESISTANCE; SERRATIA-MARCESCENS; BICINCHONINIC ACID; BACTERIAL PORIN; PROTEIN; RECONSTITUTION; ORIENTATION
abstract The molecular pathway of enrofloxacin, a fluoroquinolone antibiotic, through the outer membrane channel OmpF of Escherichia coli is investigated. High-resolution ion current fluctuation analysis reveals a strong affinity for enrofloxacin to OmpF, the highest value ever recorded for an antibiotic-channel interaction. A single point mutation in the constriction zone of OmpF, replacing aspartic acid at the 113 position with asparagine (D113N), lowers the affinity to a level comparable to other antibiotics. All-atom molecular dynamics simulations allow rationalizing the translocation pathways: wild-type OmpF has two symmetric binding sites for enrofloxacin located at each channel entry separated by a large energy barrier in the center, which inhibits antibiotic translocation. In this particular case, our simulations suggest that the ion current blockages are caused by molecules occupying either one of these peripheral binding sites. Removal of the negative charge on position 113 removes the central barrier and shifts the two peripheral binding sites to a unique central site, which facilitates translocation. Fluorescence steady-state measurements agree with the different location of binding sites for wild-type OmpF and the mutant. Our results demonstrate how a single-point mutation of the porin, and the resulting intrachannel shift of the affinity site, may substantially modify translocation.
publisher AMER CHEMICAL SOC
issn 1520-6106
year published 2010
volume 114
issue 15
beginning page 5170
ending page 5179
digital object identifier (doi) 10.1021/jp911485k
web of science category Chemistry, Physical
subject category Chemistry
unique article identifier WOS:000276563000024
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journal analysis (jcr 2019):
journal impact factor 2.857
5 year journal impact factor 2.88
category normalized journal impact factor percentile 50.629
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