PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer
authors Agarwal, V; Sardo, M; Scholz, I; Bockmann, A; Ernst, M; Meier, BH
nationality International
journal JOURNAL OF BIOMOLECULAR NMR
author keywords Solid-state NMR; Heteronuclear correlation; PAIN-CP; RESORT; Heteronuclear RESORT
keywords SOLID-STATE NMR; ANGLE-SPINNING NMR; NUCLEAR-MAGNETIC-RESONANCE; PROTEIN-STRUCTURE DETERMINATION; ESCHERICHIA-COLI THIOREDOXIN; 3D STRUCTURE DETERMINATION; ROTATING SOLIDS; CROSS-POLARIZATION; AMYLOID FIBRILS; CORRELATION SPECTROSCOPY
abstract In this article, we describe third-spin assisted heteronuclear recoupling experiments, which play an increasingly important role in measuring long-range heteronuclear couplings, in particular N-15-C-13, in proteins. In the proton-assisted insensitive nuclei cross polarization (PAIN-CP) experiment (de PaA << pe et al. in J Chem Phys 134:095101, 2011), heteronuclear polarization transfer is always accompanied by homonuclear transfer of the proton-assisted recoupling (PAR) type. We present a phase-alternating experiment that promotes heteronuclear (e.g. N-15 -> C-13) polarization transfer while simultaneously minimizing homonuclear (e.g.C-13 -> C-13) transfer (PAIN without PAR). This minimization of homonuclear polarization transfer is based on the principle of the resonant second-order transfer (RESORT) recoupling scheme where the passive proton spins are irradiated by a phase-alternating sequence and the modulation frequency is matched to an integer multiple of the spinning frequency. The similarities and differences between the PAIN-CP and this het-RESORT experiment are discussed here.
publisher SPRINGER
issn 0925-2738
year published 2013
volume 56
issue 4
beginning page 365
ending page 377
digital object identifier (doi) 10.1007/s10858-013-9756-4
web of science category Biochemistry & Molecular Biology; Spectroscopy
subject category Biochemistry & Molecular Biology; Spectroscopy
unique article identifier WOS:000323661800008
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