Image Analysis Technique as a Tool to Identify Morphological Changes in Trametes versicolor Pellets According to Exopolysaccharide or Laccase Production
authors Tavares, APM; Silva, RP; Amaral, AL; Ferreira, EC; Xavier, AMRB
nationality International
journal APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
author keywords Filamentous fungi; Pellets morphology; Image analysis; Enzyme activity; Exopolysaccharide production; Pulp and paper effluent
keywords KRAFT PULP; CORIOLUS-VERSICOLOR; SUBMERGED CULTIVATIONS; FILAMENTOUS FUNGI; GROWTH; LIGNIN; MODEL; 1-HYDROXYBENZOTRIAZOLE; POLYSACCHARIDE; MICROORGANISMS
abstract Image analysis technique was applied to identify morphological changes of pellets from white-rot fungus Trametes versicolor on agitated submerged cultures during the production of exopolysaccharide (EPS) or ligninolytic enzymes. Batch tests with four different experimental conditions were carried out. Two different culture media were used, namely yeast medium or Trametes defined medium and the addition of lignolytic inducers as xylidine or pulp and paper industrial effluent were evaluated. Laccase activity, EPS production, and final biomass contents were determined for batch assays and the pellets morphology was assessed by image analysis techniques. The obtained data allowed establishing the choice of the metabolic pathways according to the experimental conditions, either for laccase enzymatic production in the Trametes defined medium, or for EPS production in the rich Yeast Medium experiments. Furthermore, the image processing and analysis methodology allowed for a better comprehension of the physiological phenomena with respect to the corresponding pellets morphological stages.
publisher HUMANA PRESS INC
issn 0273-2289
year published 2014
volume 172
issue 4
beginning page 2132
ending page 2142
digital object identifier (doi) 10.1007/s12010-013-0675-3
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000333248000033
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journal impact factor 1.797
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