|
authors |
Goncalves, I; Abreu, AS; Matama, T; Ribeiro, A; Gomes, AC; Silva, C; Cavaco-Paulo, A |
|
journal |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
|
author keywords |
Laccase; Catechin; Trimethoprim; Sulfamethoxazole; Antimicrobial; Catheters |
|
keywords |
BACTERIAL OUTER-MEMBRANE; STAPHYLOCOCCUS-AUREUS; ANTIBACTERIAL PROPERTIES; BIOFILM RESISTANCE; TRIMETHOPRIM; ANTIBIOTICS; POLYPHENOLS; FLAVONOIDS; COMBINATION; MECHANISMS |
|
abstract |
Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N'-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing alpha-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)-TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative -85 % and gram-positive -87 %) and with poly(catechin)-TMP-SMZ (gram-negative -85 % and gram-positive -91 %). The cytotoxicity to mammalian cells was tested by indirect contact for 5 days and revealed that all the tested coatings supported more than 90 % of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections. |
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publisher |
SPRINGER |
|
issn |
0175-7598 |
|
year published |
2015 |
|
volume |
99 |
|
issue |
2 |
|
beginning page |
637 |
|
ending page |
651 |
|
digital object identifier (doi) |
10.1007/s00253-014-6128-2 |
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web of science category |
Biotechnology & Applied Microbiology |
|
subject category |
Biotechnology & Applied Microbiology |
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unique article identifier |
WOS:000348770900009
|
