|
authors |
Goncalves, I; Botelho, CM; Teixeira, A; Abreu, AS; Hilliou, L; Silva, C; Cavaco-Paulo, A |
|
journal |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
|
author keywords |
Methylparaben; Trimethoprim; Tyrosinase; Poly(hydroxybenzene); Conjugates; Antimicrobial |
|
keywords |
CATHETER-RELATED INFECTION; URINARY-TRACT INFECTIONS; AQUEOUS-SOLUTIONS; METHYL PARABEN; ACID PARABENS; HYDROXYETHYLCELLULOSE; PREVENTION; PRODUCTS; HEALTH; DERIVATIVES |
|
abstract |
Poly(hydroxybenzene)-trimethoprim conjugates were prepared using methylparaben as substrate of the oxidative enzyme tyrosinase. MALDI-TOF MS analysis showed that the enzymatic oxidation of methylparaben alone leads to the poly(hydroxybenzene) formation. In the presence of trimethoprim, the methylparaben tyrosinase oxidation leads poly(hydroxybenzene)-trimethoprim conjugates. All of these compounds were incorporated into lubricant hydroxyethyl cellulose/glycerol mixtures. Poly(hydroxybenzene)-trimethoprim conjugates were the most effective phenolic structures against the bacterial growth reducing by 96 and 97 % of Escherichia coli and Staphylococcus epidermidis suspensions, respectively (after 24 h). A novel enzymatic strategy to produce antimicrobial poly(hydroxybenzene)-antibiotic conjugates is proposed here for a wide range of applications on the biomedical field. |
|
publisher |
SPRINGER |
|
issn |
0175-7598 |
|
year published |
2015 |
|
volume |
99 |
|
issue |
10 |
|
beginning page |
4225 |
|
ending page |
4235 |
|
digital object identifier (doi) |
10.1007/s00253-015-6509-1 |
|
web of science category |
Biotechnology & Applied Microbiology |
|
subject category |
Biotechnology & Applied Microbiology |
|
unique article identifier |
WOS:000353826200007
|
