Characterization of Biocatalysts Prepared with Thermomyces lanuginosus Lipase and Different Silica Precursors, Dried using Aerogel and Xerogel Techniques
authors Barao, CE; de Paris, LD; Dantas, JH; Pereira, MM; Cardozo, L; de Castro, HF; Zanin, GM; de Moraes, FF; Soares, CMF
nationality International
author keywords Lipase; Immobilization; Sol gel; Xerogel; Aerogel; Characterization
abstract The use of lipases in industrial processes can result in products with high levels of purity and at the same time reduce pollutant generation and improve both selectivity and yields. In this work, lipase from Thermomyces lanuginosus was immobilized using two different techniques. The first involves the hydrolysis/polycondensation of a silica precursor (tetramethoxysilane (TMOS)) at neutral pH and ambient temperature, and the second one uses tetraethoxysilane (TEOS) as the silica precursor, involving the hydrolysis and polycondensation of the alkoxide in appropriate solvents. After immobilization, the enzymatic preparations were dried using the aerogel and xerogel techniques and then characterized in terms of their hydrolytic activities using a titrimetric method with olive oil and by the formation of 2-phenylethyl acetate in a transesterification reaction. The morphological properties of the materials were characterized using scanning electron microscopy, measurements of the surface area and pore size and volume, thermogravimetric analysis, and exploratory differential calorimetry. The results of the work indicate that the use of different silica precursors (TEOS or TMOS) and different drying techniques (aerogel or xerogel) can significantly affect the properties of the resulting biocatalyst. Drying with supercritical CO2 provided higher enzymatic activities and pore sizes and was therefore preferable to drying, using the xerogel technique. Thermogravimetric analysis and differential scanning calorimetry analyses revealed differences in behavior between the two biocatalyst preparations due to the compounds present.
issn 0273-2289
year published 2014
volume 172
issue 1
beginning page 263
ending page 274
digital object identifier (doi) 10.1007/s12010-013-0533-3
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000331005200023
link 24078188
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