Changes in the metabolome of lettuce leaves due to exposure to mancozeb pesticide
authors Pereira, SI; Figueiredo, PI; Barros, AS; Dias, MC; Santos, C; Duarte, IF; Gil, AM
nationality International
journal FOOD CHEMISTRY
author keywords Lettuce; Mancozeb; Metabolomics; High resolution magic angle spinning (HRMAS); Nuclear magnetic resonance (NMR); Multivariate analysis; Pesticide
keywords LACTUCA-SATIVA L.; HIGH-FIELD NMR; FUNGICIDE MANCOZEB; FOOD ANALYSIS; FRUIT; QUALITY; TOMATO; PROFILE; GRAPES; PLANTS
abstract This paper describes a proton high resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR) metabolomic study of lettuce (Lactuca sativa L.) leaves to characterise metabolic adaptations during leaf growth and exposure to mancozeb. Metabolite variations were identified through multivariate analysis and checked through spectral integration. Lettuce growth was accompanied by activation of energetic metabolism, preferential glucose use and changes in amino acids, phospholipids, ascorbate, nucleotides and nicotinatelnicotinamide. Phenylalanine and polyphenolic variations suggested higher oxidative stress at later growth stages. Exposure to mancozeb induced changes in amino acids, fumarate and malate, suggesting Krebs cycle up-regulation. In tandem disturbances in sugar, phospholipid, nucleotide and nicotinate/nicotinamide metabolism were noted. Additional changes in phenylalanine, dehydroascorbate, tartrate and formate were consistent with a higher demand for anti-oxidant defence mechanisms. Overall, lettuce exposure to mancozeb was shown to have a significant impact on plant metabolism, with mature leaves tending to be more extensively affected than younger leaves. (C) 2014 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 0308-8146
year published 2014
volume 154
beginning page 291
ending page 298
digital object identifier (doi) 10.1016/j.foodchem.2014.01.019
web of science category Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics
subject category Chemistry; Food Science & Technology; Nutrition & Dietetics
unique article identifier WOS:000332430800039
link 24518345

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