Human plasma stability during handling and storage: impact on NMR metabolomics
authors Pinto, J; Domingues, MRM; Galhano, E; Pita, C; Almeida, MD; Carreira, IM; Gil, AM
nationality International
journal ANALYST
keywords HUMAN BLOOD; H-1-NMR; SERUM; ANTICOAGULANT; SPECTROSCOPY; VALIDATION; COLLECTION; SPECTRA; URINE
abstract This work contributes to fill in some existing gaps in the knowledge of human plasma degradability during handling and storage, a paramount issue in Nuclear Magnetic Resonance (NMR) metabolomics. Regarding the comparison between heparin and EDTA anti-coagulant collection tubes, the former showed no interference of the polysaccharide, while conserving full spectral information. In relation to time/temperature conditions, room temperature was seen to have a large impact on lipoproteins and choline compounds from 2.5 hours. In addition, short-term storage at -20 degrees C was found suitable up to 7 days but, for periods up to 1 month, -80 degrees C was recommended. Furthermore, in the case of reusing plasma samples, no more than 3 consecutive freeze-thaw cycles were found advisable. Finally, the impact of long-term -80 degrees C storage (up to 2.5 years) was found almost negligible, as evaluated on a partially matched non-fasting cohort (n = 49), after having investigated the possible confounding nature of the particular non-fasting conditions employed.
publisher ROYAL SOC CHEMISTRY
issn Mar-54
year published 2014
volume 139
issue 5
beginning page 1168
ending page 1177
digital object identifier (doi) 10.1039/c3an02188b
web of science category Chemistry, Analytical
subject category Chemistry
unique article identifier WOS:000330778400039
link 24443722
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journal analysis (jcr 2017):
journal impact factor 3.864
5 year journal impact factor 3.791
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