Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
authors Taha, M; Silva, FAE; Quental, MV; Ventura, SPM; Freire, MG; Coutinho, JAP
nationality International
journal GREEN CHEMISTRY
keywords AQUEOUS BIPHASIC SYSTEMS; BOVINE SERUM-ALBUMIN; 2-PHASE SYSTEMS; TOXICITY; AGGREGATION; STABILITY; MEDIA; SALTS; WATER; SPECTROSCOPY
abstract This work reports a promising approach to the development of novel self-buffering and biocompatible ionic liquids for biological research in which the anions are derived from biological buffers (Good's buffers, GB). Five Good's buffers (Tricine, TES, CHES, HEPES, and MES) were neutralized with four suitable hydroxide bases (1-ethyl-3-methylimidazolium, tetramethylammonium, tetraethylammonium, and tetrabutylammonium) producing 20 Good's buffer ionic liquids (GB-ILs). The presence of the buffering action of the synthesized GB-ILs was ascertained by measuring their pH-profiles in water. Moreover, a series of mixed GB-ILs with wide buffering ranges were formulated as universal buffers. The impact of GB-ILs on bovine serum albumin (BSA), here used as a model protein, is discussed and compared with more conventional ILs using spectroscopic techniques, such as infrared and dynamic light scattering. They appear to display, in general, a greater stabilizing effect on the protein secondary structure than conventional ILs. A molecular docking study was also carried out to investigate on the binding sites of GB-IL ions to BSA. We further used the QSAR-human serum albumin binding model, log K(HSA), to calculate the binding affinity of some conventional ILs/GB-ILs to HSA. The toxicity of the GB and GB-ILs was additionally evaluated revealing that they are non-toxic against Vitro fischeri. Finally, the GB-ILs were also shown to be able to form aqueous biphasic systems when combined with aqueous solutions of inorganic or organic salts, and we tested their extraction capability for BSA. These systems were able to extract BSA with an outstanding extraction efficiency of 100% in a single step for the GB-IL-rich phase, and, as a result, the use of GB-IL-based ABS for the separation and extraction of other added-value biomolecules is highly encouraging and worthy of further investigation.
publisher ROYAL SOC CHEMISTRY
issn 1463-9262
year published 2014
volume 16
issue 6
beginning page 3149
ending page 3159
digital object identifier (doi) 10.1039/c4gc00328d
web of science category Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY
subject category Chemistry; Science & Technology - Other Topics
unique article identifier WOS:000336836100027
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journal analysis (jcr 2017):
journal impact factor 8.586
5 year journal impact factor 8.717
category normalized journal impact factor percentile 91.733
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