Evaluating Self-buffering Ionic Liquids for Biotechnological Applications
authors Lee, SY; Vicente, FA; Silva, FAE; Sintra, TE; Taha, M; Khoiroh, I; Coutinho, JAP; Show, PL; Ventura, SPM
nationality International
journal ACS SUSTAINABLE CHEMISTRY & ENGINEERING
author keywords Good's buffer ionic liquids; Self-buffering GB-ILs; Aqueous biphasic systems; Enzyme-stabilizing systems; Biocatalysis and bioseparation
keywords AQUEOUS BIPHASIC SYSTEMS; FISCHERI MARINE-BACTERIA; BOVINE SERUM-ALBUMIN; ENHANCED EXTRACTION; BIOLOGICAL-RESEARCH; AQUATIC ORGANISMS; GOODS BUFFERS; LIPASE; TOXICITY; PURIFICATION
abstract A new range of Goods buffer ionic liquids (GB-ILs), displaying simultaneously the properties of ionic liquids and Goods buffers, were synthesized by a combination of Goods buffer anions (MOPSO, BES, TAPSO, and CAPSO) and tetrabutylammonium, tetrabutylphosphonium, and cholinium cations via an acidbase neutralization reaction. The activity and stability of a lipolytic enzyme from Pseudomonas cepacia in aqueous solutions of these buffers were evaluated, and the results show their advantage as media for enzymatic reactions when compared to conventional phosphate buffers. Moreover aqueous biphasic systems (ABS) composed by these GB-ILs and potassium citrate were investigated and shown to be highly effective and selective for the partitioning of the lipolytic enzyme into the GB-IL-rich phase. The results allow for the development of an efficient and biocompatible process combining the self-buffering and enzyme-stabilizing properties of the GB-ILs in the reaction step, with the advantages of GB-ILs as extraction solvents in ABS.
publisher AMER CHEMICAL SOC
issn 2168-0485
year published 2015
volume 3
issue 12
beginning page 3420
ending page 3428
digital object identifier (doi) 10.1021/acssuschemeng.5b01155
web of science category Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical
subject category Chemistry; Science & Technology - Other Topics; Engineering
unique article identifier WOS:000366153700049

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