Enhanced extraction of proteins using cholinium-based ionic liquids as phase-forming components of aqueous biphasic systems
authors Quental, MV; Caban, M; Pereira, MM; Stepnowski, P; Coutinho, JAP; Freire, MG
nationality International
journal BIOTECHNOLOGY JOURNAL
author keywords Bovine serum albumin; Cholinium-based aqueous biphasic system; Efficient extraction; Ionic liquid; Purification
keywords BOVINE SERUM-ALBUMIN; 2-PHASE SYSTEMS; POLYETHYLENE-GLYCOL; EXCHANGE CHROMATOGRAPHY; MOLECULAR-INTERACTIONS; MONOCLONAL-ANTIBODIES; BIOLOGICAL-RESEARCH; PURIFICATION; SEPARATION; STABILITY
abstract Aqueous biphasic systems (ABS) composed of ionic liquids (ILs) are promising platforms for the extraction and purification of proteins. In this work, a series of alternative and biocompatible ABS composed of cholinium-based ILs and polypropylene glycol were investigated. The respective ternary phase diagrams, tie-lines, tie-line lengths and critical points were determined at 25 degrees C. The extraction performance of these systems for commercial bovine serum albumin (BSA) was then evaluated. The stability of BSA at the IL-rich phase was ascertained by size exclusion high-performance liquid chromatography and Fourier transform infrared spectroscopy. Appropriate ILs lead to the complete extraction of BSA for the IL-rich phase, in a single step, while maintaining the protein's native conformation. Furthermore, to evaluate the performance of these systems when applied to real matrices, the extraction of BSA from bovine serum was additionally carried out, revealing that the complete extraction of BSA was maintained and achieved in a single step. The remarkable extraction efficiencies obtained are far superior to those observed with typical polymer-based ABS. Therefore, the proposed ABS may be envisaged as a more effective and biocompatible approach for the separation and purification of other value-added proteins.
publisher WILEY-V C H VERLAG GMBH
issn 1860-6768
year published 2015
volume 10
issue 9
beginning page 1457
ending page 1466
digital object identifier (doi) 10.1002/biot.201500003
web of science category Biochemical Research Methods; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000362724300011
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