Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems
authors Ferreira, AM; Faustino, VFM; Mondal, D; Coutinho, JAP; Freire, MG
nationality International
journal JOURNAL OF BIOTECHNOLOGY
author keywords Aqueous biphasic system; Ionic liquids; Adjuvants; Antibody; Extraction; Purification
keywords 2-PHASE SYSTEMS; MONOCLONAL-ANTIBODIES; PROTEINS; OPTIMIZATION; SEPARATION; STABILITY; RECOVERY; IGG
abstract Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5 wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals. (C) 2016 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0168-1656
year published 2016
volume 236
beginning page 166
ending page 175
digital object identifier (doi) 10.1016/j.jbiotec.2016.08.015
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000384852600017
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 2.533
5 year journal impact factor 2.843
category normalized journal impact factor percentile 58.696
dimensions (citation analysis):
altmetrics (social interaction):



 


Apoio

1suponsers_list_ciceco.jpg