Polyhydroxyalkanoates-accumulating bacteria isolated from activated sludge acclimatized to hardwood sulphite spent liquor
authors Ferreira, AM; Queiros, D; Gagliano, MC; Serafim, LS; Rossetti, S
nationality International
journal ANNALS OF MICROBIOLOGY
author keywords Polyhydroxyalkanoates; Mixed microbial culture; Hardwood sulfite spent liquor; Microbial isolation
keywords MIXED MICROBIAL CULTURES; CHAIN FATTY-ACIDS; SP-NOV.; PHA PRODUCTION; DEGRADING BACTERIUM; WASTE-WATER; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE); IDENTIFICATION; BIOSYNTHESIS; PULP
abstract This work aimed to isolate and characterize organisms able to store polyhydroxyalkanoates (PHAs), from a mixed microbial culture (MMC) selected under feast and famine conditions, using hardwood sulfite spent liquor (HSSL), a by-product of the pulp and paper industry, as the carbon source. The MMC was able to accumulate PHA from the different HSSL carbon components (acetic acid, xylose and lignosulfonates) with a PHA storage content during the reactor operation of 54.2 +/- 10.6 % PHB. In order to clarify the MMC composition, isolation was performed from the MMC, using HSSL or its main components. Three isolates were identified: Rhodococcus spp., Pseudomonas spp. and Klebsiella spp. All isolates were able to grow and accumulate PHAs in harsh conditions of HSSL using xylose, the second most abundant sugar in nature. Rhodococcus and Pseudomonas isolates were able to accumulate a homopolymer PHB, whereas Klebsiella accumulated the copolymer P(HB-co-HV), with a composition of 96 % HB and 4 % HV. 16S rDNA clonal analysis was performed to identify the unculturable fraction of the MMC, and members of Novosphingobium spp., Sphingobium spp. and Pleomorphomonas spp., were found to be related to PHA production. The capability of the isolated bacteria to simultaneously consume xylose and accumulate PHAs offers a possibility for further studies and routes for the valorization of xylose-rich by-products.
publisher SPRINGER
issn 1590-4261
year published 2016
volume 66
issue 2
beginning page 833
ending page 842
digital object identifier (doi) 10.1007/s13213-015-1169-z
web of science category Biotechnology & Applied Microbiology; Microbiology
subject category Biotechnology & Applied Microbiology; Microbiology
unique article identifier WOS:000375051100031
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journal impact factor 1.407
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