Timesaving microwave assisted synthesis of insulin amyloid fibrils with enhanced nanofiber aspect ratio
authors Carvalho, T; Pinto, RJB; Martins, MA; Silvestre, AJD; Freire, CSR
nationality International
journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
author keywords Insulin fibrils; Microwave irradiation; Aspect ratio; Timesaving method
keywords THIOFLAVIN T; PROTEIN; FIBRILLATION; POLYMORPHISM; AGGREGATION; MECHANISMS; MORPHOLOGY; NANOWIRES; TOXICITY
abstract Insulin amyloid fibrils with enhanced aspect ratio, were prepared using a timesaving microwave assisted (MW) methodology, reducing the incubation time from 13 to 2 h. The fibrillation process was followed indirectly by Thioflavin T Fluorescence and UV-vis analysis, by measuring the amount of beta-sheets formed and the insulin present in solution, respectively. TEM and AFM analysis revealed that the insulin fibrils obtained through the MW method, have very similar lengths but are much thinner than the ones obtained using the conventional method (CM). Additionally, it was verified that the nature of the peptides present in the final insulin fibrils was not affected by microwave irradiation. These morphological differences might reflect on noticeably enhanced mechanical and optical properties that can exploited on the development of advanced bionanomaterials. (C) 2016 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0141-8130
year published 2016
volume 92
beginning page 225
ending page 231
digital object identifier (doi) 10.1016/j.ijbiomac.2016.07.008
web of science category Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science
subject category Biochemistry & Molecular Biology; Chemistry; Polymer Science
unique article identifier WOS:000386402900028
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journal impact factor 3.909
5 year journal impact factor 3.929
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