|
authors |
Goncalves, I; Nunes, C; Mendes, S; Martins, LO; Ferreira, P; Coimbra, MA |
|
nationality |
International |
|
journal |
CARBOHYDRATE POLYMERS |
|
author keywords |
Chitosan-genipin films; Decolorization; Laccase; Mediator; Hydrogen peroxide; Active materials |
|
keywords |
BOVINE SERUM-ALBUMIN; CORN FIBER GUM; ANTIOXIDANT ACTIVITY; HYDROGEN-PEROXIDE; BACILLUS-SUBTILIS; STABILITY; BACTERIAL; MEDIATOR; CYTOCOMPATIBILITY; GLYCEROL |
|
abstract |
Chitosan-genipin films present a bluish-green color due to the conjugated double bonds formed when monomeric or dimeric genipin residues bridge chitosan. This phenomenon limits their use when colorless materials are required. In this work, a two-step oxidation strategy was developed aiming to remove color from chitosan-genipin films while preserving their functional properties. A combined system using the recombinant CotA laccase from Bacillus subtilis mediated by 2,2'-azinobis-(3-ethylbenzothiazoline6-sulphonic acid) (ABTS) followed by a H2O2 oxidation step was settled. ABTS boosted the laccase performance resulting in light brown chitosan-genipin films that were further decolorized upon immersion in a 5% H2O2 solution at pH 11.0 and 40 degrees C for 30 min. The applied methodology leads to films that sustain both acidic stability and antioxidant capacity of the pristine films. Overall, the combined CotA laccase-ABTS/hydrogen peroxide developed methodology efficiently produce active and decolorized chitosan-genipin films with potential for application as eco-friendly transparent material which may have application as food packaging. (C) 2017 Elsevier Ltd. All rights reserved. |
|
publisher |
ELSEVIER SCI LTD |
|
issn |
0144-8617 |
|
year published |
2017 |
|
volume |
175 |
|
beginning page |
628 |
|
ending page |
635 |
|
digital object identifier (doi) |
10.1016/j.carbpol.2017.08.029 |
|
web of science category |
Chemistry, Applied; Chemistry, Organic; Polymer Science |
|
subject category |
Chemistry; Polymer Science |
|
unique article identifier |
WOS:000411112500072
|
