Overall biochemical changes in bacteria photosensitized with cationic porphyrins monitored by infrared spectroscopy
authors Moreirinha, C; Alves, E; Faustino, MAF; Cunha, A; Delgadillo, I; Neves, MGPMS; Almeida, A
nationality International
journal FUTURE MEDICINAL CHEMISTRY
author keywords Escherichia coli; lipopolysaccharides; oxidative damage; photodynamic; photosensitizer; Staphylococcus warneri
keywords PHOTODYNAMIC INACTIVATION; ESCHERICHIA-COLI; FTIR SPECTROSCOPY; TOLUIDINE BLUE; MEMBRANE PHOSPHOLIPIDS; STAPHYLOCOCCUS-WARNERI; METHYLENE-BLUE; MECHANISMS; OXIDATION; PROTEINS
abstract Photodynamic inactivation of micro-organisms is a promising nonantibiotic multitarget approach to treat localized and superficial infections through oxidative stress. Herein, the changes occurring on major cellular components of Escherichia coli and Staphylococcus warneri, induced by photosensitization with cationic porphyrins (Tri-Py+-Me-PF and Tetra-Py+-Me) and white light, were monitored by infrared spectroscopy. Results: In E. coli, most of the changes occurred on proteins and lipids, suggesting a key effect on lipopolysaccharides in the first irradiation times. In S. warneri, proteins were the major molecular targets of oxidative damage but phospholipids and polysaccharides were also affected. Conclusion: Infrared spectroscopy is a very interesting tool to monitor biochemical changes induced by photosensitization in bacteria and also to infer on its mechanism of action.
publisher FUTURE SCI LTD
issn 1756-8919
year published 2016
volume 8
issue 6
beginning page 613
ending page 628
digital object identifier (doi) 10.4155/fmc-2015-0008
web of science category Chemistry, Medicinal
subject category Pharmacology & Pharmacy
unique article identifier WOS:000374769000003
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