Impact of physical exercise on visceral adipose tissue fatty acid profile and inflammation in response to a high-fat diet regimen
authors Rocha-Rodrigues, S; Rodriguez, A; Goncalves, IO; Moreira, A; Maciel, E; Santos, S; Domingues, MR; Fruhbeck, G; Ascensao, A; Magalhaes, J
nationality International
journal INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
author keywords Endurance training; Visceral adiposity; Fatty acids; TNF-alpha; IL-10
keywords INDUCED INSULIN-RESISTANCE; OBESE MICE; GENE-EXPRESSION; LINOLEIC-ACID; OXIDATIVE STRESS; LIPID-METABOLISM; RATS; MACROPHAGES; LIVER; ALPHA
abstract Purpose: Studies associate specific fatty-acids (FA) with the pathophysiology of inflammation. We aimed to analyze the impact of exercise on adipose tissue FA profile in response to a high-fat diet (HFD) and to ascertain whether these exercise-induced changes in specific FA have repercussions on obesity-related inflammation. Methods: Sprague-Dawley rats were assigned into sedentary, voluntary physical-activity (VPA) and endurance training (ET) groups fed a standard (S, 35kcal% fat) or high-fat (71kcal% fat) diets. VPA-animals had unrestricted access to wheel-running. After 9-wks, ET-animals engaged a running protocol for 8-wks, while maintained dietary treatments. The FA content in epididymal white-adipose tissue (eWAT) triglycerides was analyzed by gas-chromatography and the expression of inflammatory markers was determined using RT-qPCR, Western and slot blotting. Results: Eight-wks of ET reversed obesity-related anatomical features. HFD increased plasma tumor necrosis factor (TNF)-alpha content and eWAT monocyte chemoattractant protein (MCP)-1 protein expression. HFD decreased eWAT content of saturated FA and monounsaturated FA, while increased linoleic acid and prostaglandin E2 (PGE2) levels in eWAT. VPA decreased visceral adiposity, adipocyte size and MCP-1 in HFD-fed animals. The VPA and ET interventions diminished palmitoleic acid and increased linoleic acid in HFD-fed groups. Moreover, both interventions increased PGE2 levels in standard diet-fed groups and decreased in HFD. ET increased eWAT fatty acid desaturase 1 (FADS1) and elongase 5 (ELOVL5) protein content in both diet types. ET reduced eWAT inflammatory markers (TNF-alpha, IL-6), macrophage recruitment (MCP-1 and F4/80) and increased IL-10/TNF-alpha ratio in plasma and in eWAT in both diet types. Conclusions: Exercise induced FA-specific changes independently of dietary FA composition, but only ET attenuated the inflammatory response in VAT of HFD-fed rats. Moreover, the exercise-induced FA changes did not correlate with the inflammatory response in VAT of rats submitted to HFD.
publisher PERGAMON-ELSEVIER SCIENCE LTD
issn 1357-2725
year published 2017
volume 87
beginning page 114
ending page 124
digital object identifier (doi) 10.1016/j.bioce1.2017.04.008
web of science category Biochemistry & Molecular Biology; Cell Biology
subject category Biochemistry & Molecular Biology; Cell Biology
unique article identifier WOS:000403528400014
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  impact metrics
journal analysis (jcr 2017):
journal impact factor 3.247
5 year journal impact factor 3.813
category normalized journal impact factor percentile 53.501
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