Gold nanoparticles and bioconjugation: a pathway for proteomic applications
authors Couto, C; Vitorino, R; Daniel-da-Silva, AL
nationality International
journal CRITICAL REVIEWS IN BIOTECHNOLOGY
author keywords Biosensor; gold nanoparticles; synthesis; functionalization; protein; ligand; protein enrichment; mass spectrometry
keywords MASS-SPECTROMETRY; ULTRASENSITIVE DETECTION; PROTEIN CRYSTALLIZATION; COLORIMETRIC SENSOR; METAL NANOPARTICLES; CHEMICAL-DETECTION; COLLOIDAL GOLD; PARTICLE-SIZE; SEEDED GROWTH; SHAPE CONTROL
abstract In the last few years gold nanoparticles (AuNPs) became extremely interesting materials due to their enhanced optical, chemical and electrical properties. With the intention of taking advantage of those properties, the use of AuNPs has spread into a wide variety of areas such as physics, chemistry, biology, industry and medicine. More interestingly, their ability to form robust conjugates with biomolecules has given proteomics a new tool to improve aspects where the current methods to study proteins and their interactions in living cells cannot achieve the success required. In this review we present some of the current methods for AuNPs synthesis, the tailoring of their surface with ligands to improve stability and strategies to conjugate with biomolecules. Lastly, we also discuss their application in proteomic methods and recent developments in clinical diagnosis.
publisher TAYLOR & FRANCIS LTD
issn 0738-8551
isbn 1549-7801
year published 2017
volume 37
issue 2
beginning page 238
ending page 250
digital object identifier (doi) 10.3109/07388551.2016.1141392
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000392639500010
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  impact metrics
journal analysis (jcr 2017):
journal impact factor 5.239
5 year journal impact factor 6.019
category normalized journal impact factor percentile 87.888
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