The influence of surface modified poly(L-lactic acid) films on the differentiation of human monocytes into macrophages
authors Correia, CR; Gaifem, J; Oliveira, MB; Silvestre, R; Mano, JF
nationality International
journal BIOMATERIALS SCIENCE
keywords FOREIGN-BODY REACTION; MATRIX METALLOPROTEINASES; POLARIZATION; BIOMATERIALS; SCAFFOLDS; INFLAMMATION; CYTOKINES; BONE; REGENERATION; ADHESION
abstract Macrophages play a crucial role in the biological performance of biomaterials, as key factors in defining the optimal inflammation-healing balance towards tissue regeneration and implant integration. Here, we investigate how different surface modifications performed on poly(L-lactic acid) (PLLA) films would influence the differentiation of human monocytes into macrophages. We tested PLLA films without modification, surface-modified by plasma treatment (pPLLA) or by combining plasma treatment with different coating materials, namely poly(L-lysine) and a series of proteins from the extracellular matrix: collagen I, fibronectin, vitronectin, laminin and albumin. While all the tested films are non-cytotoxic, differences in cell adhesion and morphology are observed. Monocyte-derived macrophages (MDM) present a more rounded shape in non-modified films, while a more elongated phenotype is observed containing filopodia-like and podosome-like structures in all modified films. No major differences are found for the expression of HLA-DR+/CD80(+) and CD206(+)/CD163(+) surface markers, as well as for the ability of MDM to phagocytize. Interestingly, MDM differentiated on pPLLA present the highest expression of MMP9. Upon differentiation, MDM in all surface modified films present lower amounts of IL-6 and IL-10 compared to non-modified films. After stimulating MDM with the potent pro-inflammatory agent LPS, pPLLA and poly(L-lysine) and fibronectin-modified films reveal a significant reduction in IL-6 secretion, while the opposite effect is observed with IL-10. Of note, in comparison to non-modified films, all surface modified films induce a significant reduction of the IL-6/IL-10 ratio, a valuable prognosticator of the pro-versus anti-inflammatory balance. These findings provide important insights into MDM-biomaterial interactions, while strengthening the need for designing immune-informed biomaterials.
publisher ROYAL SOC CHEMISTRY
issn 2047-4830
isbn 2047-4849
year published 2017
volume 5
issue 3
beginning page 551
ending page 560
digital object identifier (doi) 10.1039/c6bm00920d
web of science category Materials Science, Biomaterials
subject category Materials Science
unique article identifier WOS:000395886300020
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journal analysis (jcr 2019):
journal impact factor 6.183
5 year journal impact factor 5.468
category normalized journal impact factor percentile 85.526
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