Impact of the Pd(2)Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
authors Lamego, I; Marques, MPM; Duarte, IF; Martins, AS; Oliveira, H; Gil, AM
nationality International
journal JOURNAL OF PROTEOME RESEARCH
author keywords palladium anticancer drugs; osteosarcoma; osteoblasts; metabolomics; NMR
keywords HUMAN BREAST-CANCER; PLATINUM COMPLEXES; COLORIMETRIC ASSAY; ANTICANCER AGENTS; DNA INTERACTIONS; ANTITUMOR DRUGS; CARCINOMA CELLS; TUMOR-CELLS; CISPLATIN; PALLADIUM(II)
abstract A metabolomics study of Pd(2)Sperrnine(Spm) on osteosarcoma MG-63 and oSteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd(2)Spm indticed lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic:amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd(2)Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative.mechanisms (e.g., glutathione,,inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd(2)Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd(2)Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd(2)Spm.
publisher AMER CHEMICAL SOC
issn 1535-3893
isbn 1535-3907
year published 2017
volume 16
issue 4
beginning page 1773
ending page 1783
digital object identifier (doi) 10.1021/acs.jproteome.7b00035
web of science category Biochemical Research Methods
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000398985700035
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journal analysis (jcr 2017):
journal impact factor 3.950
5 year journal impact factor 4.033
category normalized journal impact factor percentile 82.911
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