Metabolic Reprogramming of Macrophages Exposed to Silk, Poly(lactic-co-glycolic acid), and Silica Nanoparticles
authors Saborano, R; Wongpinyochit, T; Totten, JD; Johnston, BF; Seib, FP; Duarte, IF
nationality International
journal ADVANCED HEALTHCARE MATERIALS
author keywords macrophages; NMR metabolomics; PLGA nanoparticles; silica nanoparticles; silk nanoparticles
keywords NMR-BASED METABONOMICS; DRUG-DELIVERY; IN-VITRO; BIOMEDICAL APPLICATIONS; CARBON NANOTUBES; BREAST-CANCER; ITACONIC ACID; ACTIVATION; GENERATION; EXPRESSION
abstract Monitoring macrophage metabolism in response to nanoparticle exposure provides new insights into biological outcomes, such as inflammation or toxicity, and supports the design of tailored nanomedicines. This paper describes the metabolic signature of macrophages exposed to nanoparticles ranging in diameter from 100 to 125 nm and made from silk, poly(lactic-co-glycolic acid) or silica. Nanoparticles of this size and type are currently at various stages of preclinical and clinical development for drug delivery applications. H-1 NMR analysis of cell extracts and culture media is used to quantify the changes in the intracellular and extracellular metabolomes of macrophages in response to nanoparticle exposure. Increased glycolytic activity, an altered tricarboxylic acid cycle, and reduced ATP generation are consistent with a proinflammatory phenotype. Furthermore, amino acids possibly arising from autophagy, the creatine kinase/phosphocreatine system, and a few osmolytes and antioxidants emerge as important players in the metabolic reprogramming of macrophages exposed to nanoparticles. This metabolic signature is a common response to all nanoparticles tested; however, the direction and magnitude of some variations are clearly nanoparticle specific, indicating material-induced biological specificity. Overall, metabolic reprogramming of macrophages can be achieved with nanoparticle treatments, modulated through the choice of the material, and monitored using H-1 NMR metabolomics.
publisher WILEY
issn 2192-2640
isbn 2192-2659
year published 2017
volume 6
issue 14
digital object identifier (doi) 10.1002/adhm.201601240
web of science category Engineering, Biomedical; Nanoscience & Nanotechnology; Materials Science, Biomaterials
subject category Engineering; Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000405801800001
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journal analysis (jcr 2017):
journal impact factor 5.609
5 year journal impact factor 5.849
category normalized journal impact factor percentile 83.503
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