Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns
authors Leite, AJ; Oliveira, MB; Caridade, SG; Mano, JF
nationality International
journal ADVANCED FUNCTIONAL MATERIALS
author keywords array-based screening; combinatorial biomaterials screening; flat platforms; high-throughput screening; nanocomposites; nondestructive image analysis
keywords STEM-CELL FATE; TISSUE-ENGINEERING APPLICATIONS; GLASS-CERAMIC NANOPARTICLES; DYNAMIC-MECHANICAL ANALYSIS; IN-VITRO CHARACTERIZATION; HYALURONIC-ACID; BIOACTIVE GLASS; HIGH-THROUGHPUT; REGENERATIVE MEDICINE; COMPOSITE SCAFFOLDS
abstract Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments.
publisher WILEY-V C H VERLAG GMBH
issn 1616-301X
isbn 1616-3028
year published 2017
volume 27
issue 28
digital object identifier (doi) 10.1002/adfm.201701219
web of science category Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter
subject category Chemistry; Science & Technology - Other Topics; Materials Science; Physics
unique article identifier WOS:000406183100015
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  impact metrics
journal impact factor (jcr 2016): 12.124
5 year journal impact factor (jcr 2016): 12.362
category normalized journal impact factor percentile (jcr 2016): 92.921
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