A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints
authors Bastos, V; Duarte, IF; Santos, C; Oliveira, H
nationality International
journal JOURNAL OF NANOPARTICLE RESEARCH
author keywords Cell cycle; Citrate-coated silver nanoparticles; Nanotoxicity; Raw 264.7. ROS and Viability; Health effects
keywords IN-VITRO; PARTICLE DISSOLUTION; CELLULAR-RESPONSES; SCAVENGER RECEPTOR; TOXICITY; NANOTOXICITY; GENOTOXICITY; MACROPHAGES; APOPTOSIS; SILICA
abstract Citrate-coated silver nanoparticles (citrate-AgNPs) are among the most commonly used nanomaterials, widely present in industrial and biomedical products. In this study, the cytotoxicity of 30-nm citrate-AgNPs on the macrophage cell line RAW 264.7 was evaluated, using a battery of cytotoxicity endpoints (viability, oxidative stress, and cytostaticity/clastogenicity), at 24 and 48 h of exposure. Citrate-AgNPs decreased cell proliferation and viability only at 75 mu g/mL, suggesting a low sensitivity of RAW cells to lower doses of these AgNPs. After 24 h of exposure, ROS content decreased in cells exposed to 60 mu g/mL AgNPs (IC20 value), corroborating the high tolerance of these cells to citrateAgNPs. However, these cells suffered an impairment of the cell cycle, shown by an increase at the sub-G1 phase. This increase of the sub-G1 population was correlated with an increase of DNA fragmentation, suggesting an increase of apoptosis. Thus, our data are important to understand the effects of low concentrations (IC20) of citrate-AgNPs on in vitro vital macrophage functions.
publisher SPRINGER
issn 1388-0764
isbn 1572-896X
year published 2017
volume 19
issue 5
digital object identifier (doi) 10.1007/s11051-017-3855-1
web of science category Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
subject category Chemistry; Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000411830200001
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journal analysis (jcr 2017):
journal impact factor 2.127
5 year journal impact factor 2.333
category normalized journal impact factor percentile 45.421
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