Genotoxicity of citrate-coated silver nanoparticles to human keratinocytes assessed by the comet assay and cytokinesis blocked micronucleus assay
authors Bastos, V; Duarte, IF; Santos, C; Oliveira, H
nationality International
journal ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
author keywords Silver nanoparticles (AgNPs); CBMN; Comet assay; Genotoxicity; HaCaTcells; Nanotoxicology; Skin; Viability
keywords CELL-CYCLE ARREST; DNA-DAMAGE; IN-VITRO; DIFFERENT SIZES; LINE; CYTOTOXICITY; TOXICITY; NANOTOXICITY; PARTICLES; APOPTOSIS
abstract Silver nanoparticles (AgNPs) are widely used in industrial, cosmetic, and biomedical products, and humans are frequently exposed to these products through the skin. It is widely recognized that the characteristics of AgNPs (e. g., size, coating) may influence their cytotoxic effects, but their correlation with DNA damage and mitotic disorders remains poorly explored. In this study, human keratinocytes (HaCaT cell line) were exposed to well-characterized 30 nm AgNPs coated with citrate, and their effects on viability, DNA fragmentation (assessed by the comet assay), and micronuclei (MNi) induction (assessed by the cytokinesis-block micronucleus cytome assays, CBMN) were investigated. The results showed that 10 and 40 mu g/mL AgNPs decreased cell proliferation and viability, and induced a significant genetic damage. This was observed by an increase of DNA amount in comet tail, which linearly correlated with dose and time of exposure. Also, cytostaticity (increase of mononucleated cells) and MNi rates increased in treated cells. In contrast, no significant changes were observed in nucleoplasmatic bridges (NPBs) or nuclear buds (NBUDs), although NBUDs tended to increase in all conditions and periods. The cytostatic effects on HaCaT cells were also shown by the decrease of their nuclear division index. Thus, both comet and CBMN assays supported the observation that citrate-AgNPs induced genotoxic effects on HaCaTcells. Considering that AgNPs are present in a vast number of consumer products and also in multiple nanomedicine skin applications and formulations, more research is needed to determine the properties that confer less toxicity of AgNPs to different cell lines.
publisher SPRINGER HEIDELBERG
issn 0944-1344
isbn 1614-7499
year published 2017
volume 24
issue 5
beginning page 5039
ending page 5048
digital object identifier (doi) 10.1007/s11356-016-8240-6
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
unique article identifier WOS:000397007200071
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