Biocompatibility and antimicrobial activity of biphasic calcium phosphate powders doped with metal ions for regenerative medicine
authors Marques, CF; Olhero, S; Abrantes, JCC; Marote, A; Ferreira, S; Vieira, SI; Ferreira, JMF
nationality International
journal CERAMICS INTERNATIONAL
author keywords Biphasic calcium phosphates; Ionic substitution; Antimicrobial; Biocompatibility
keywords BETA-TRICALCIUM PHOSPHATE; HYDROXYAPATITE COATINGS; BIOLOGICAL-PROPERTIES; BONE-FORMATION; IN-VIVO; ZINC; ANTIBACTERIAL; COPPER; MAGNESIUM; CERAMICS
abstract Biphasic [hydroxyapatite (HA) + beta-tricalcium phosphate (beta-TCP)] calcium phosphate powders with partial substitutions of Ca2+ by silver, copper, zinc and strontium ions were synthesized by precipitation. The aim was to improve the overall biological performance (antimicrobial effect and stimulated osteoblasts proliferation) of the bone graft materials made thereof. The prepared powders were calcined and characterized by different techniques including X-ray diffraction (XRD), elemental analysis, thermo gravimetric and differential thermal analysis (TG-DTA), Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). Rietveld refinement was used for quantitative phase analysis. The in vitro biocompatibility and the antibacterial activity of the powders were evaluated through the resazurin viability test with MG63 cells, and against Escherichia coli and Staphylococcus aureus, respectively. The quantitative elemental analysis revealed that only strontium ions have been fully incorporated into both HA and beta-TCP phases. The in vitro biocompatibility with pre-osteoblastic cells was proved for all synthesized powders, while only the silver-doped powders exhibited antimicrobial activity against S. Aureus. The information gathered is of high relevance for designing improved calcium phosphate bioceramics.
publisher ELSEVIER SCI LTD
issn 0272-8842
year published 2017
volume 43
issue 17
beginning page 15719
ending page 15728
digital object identifier (doi) 10.1016/j.ceramint.2017.08.133
web of science category Materials Science, Ceramics
subject category Materials Science
unique article identifier WOS:000413175300157
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journal impact factor 3.057
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