A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid's proteome
authors Trindade, F; Bastos, P; Leite-Moreira, A; Manadas, B; Ferreira, R; Soares, SF; Daniel-da-Silva, AL; Falcao-Pires, I; Vitorino, R
nationality International
journal ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
author keywords Pericardial fluid; Proteomics; Magnetic nanoparticles
keywords EFFUSIONS; NETWORKS; PROTEINS; SWATH
abstract Owing to their close proximity, pericardial fluid (PF)'s proteome may mirror the pathophysiological status of the heart. Despite this diagnosis potential, the knowledge of PF's proteome is scarce. Large amounts of albumin hamper the characterization of the least abundant proteins in PR. Aiming to expand PF's proteome and to validate the technique for future applications, we have fractionated and characterized the PF, using N-(trimethoxysilylpropyl)ethylenediamine triacetic acid (EDTA)-functionalized magnetic nanoparticles (NPs@EDTA) followed by a GeLC-MS/MS approach. Similarly to an albumin depletion kit, NPs@EDTA-based fractionation was efficient in removing albumin. Both methods displayed comparable inter-individual variability, but NPs@EDTA outperformed the former with regard to the protein dynamic range as well as to the monitoring of biological processes. Overall, 565 proteins were identified, of which 297 (>50%) have never been assigned to PR. Moreover, owing to this method's good proteome reproducibility, affordability, rapid automation and high binding ability of NP@EDTA, it bears a great potential towards future clinical application. (C) 2017 Elsevier Inc. All rights reserved.
publisher ELSEVIER SCIENCE INC
issn Mar-61
year published 2017
volume 634
beginning page 1
ending page 10
digital object identifier (doi) 10.1016/j.abb.2017.09.016
web of science category Biochemistry & Molecular Biology; Biophysics
subject category Biochemistry & Molecular Biology; Biophysics
unique article identifier WOS:000417227500001
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journal analysis (jcr 2017):
journal impact factor 3.118
5 year journal impact factor 3.210
category normalized journal impact factor percentile 62.094
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