Separation of immunoglobulin G using aqueous biphasic systems composed of cholinium-based ionic liquids and poly(propylene glycol)
authors Ramalho, CC; Neves, CMSS; Quental, MV; Coutinho, JAP; Freire, MG
nationality International
journal JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
author keywords aqueous two-phase systems; bioseparations; downstream; liquid-liquid extraction; pharmaceuticals
keywords BOVINE SERUM-ALBUMIN; 2-PHASE SYSTEMS; MONOCLONAL-ANTIBODIES; ENHANCED EXTRACTION; POLYETHYLENE-GLYCOL; BIOLOGICAL-RESEARCH; PROTEIN SEPARATION; GOODS BUFFERS; HUMAN PLASMA; HUMAN-IGG
abstract BACKGROUNDThe use of antibodies, such as immunoglobulin G (IgG), has seen a significant growth in recent decades for biomedical and research purposes. However, antibodies are high cost biopharmaceuticals, for which the development of alternative and cost-effective purification strategies is still in high demand. RESULTSAqueous biphasic systems (ABS) composed of poly(propylene glycol) (PPG) and cholinium-based ionic liquids (ILs) were investigated for the separation of IgG. The ABS phase diagrams were determined whenever required. Initial studies with commercial IgG were carried out, followed by IgG separation from rabbit serum. In all ABS, IgG preferentially partitions to the IL-rich phase, unveiling preferential interactions between IgG and ILs. Extraction efficiencies ranging between 93% and 100%, and recovery yields ranging between 20% and 100%, were obtained for commercial IgG. Two of the best and two of the worst identified ABS were then evaluated for their performance to separate IgG from rabbit serum, where extraction efficiencies of 100% and recovery yields >80% were obtained. Under the best conditions studied, IgG with a purity level of 49% was obtained in a single-step. After an ultrafiltration step applied to the best ABS, allowing the IgG recovery from the IL-rich phase, the IgG purity level increased to 66%. This purity level of IgG is higher than those previously reported using other IL-polymer ABS. CONCLUSIONIgG preferentially migrates to the IL-rich phase in ABS formed by ILs and polymers, allowing the design of effective separation systems for its recovery from serum samples. (c) 2018 Society of Chemical Industry
publisher WILEY
issn 0268-2575
year published 2018
volume 93
issue 7
beginning page 1931
ending page 1939
digital object identifier (doi) 10.1002/jctb.5594
web of science category Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical
subject category Biotechnology & Applied Microbiology; Chemistry; Engineering
unique article identifier WOS:000435087800013
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