authors |
Ferreira, AM; Passos, H; Okafuji, A; Tavares, APM; Ohno, H; Freire, MG; Coutinho, JAP |
nationality |
International |
journal |
GREEN CHEMISTRY |
keywords |
AMMONIUM-BASED ZWITTERIONS; IONIC LIQUIDS; POLYETHYLENE-GLYCOL; 2-PHASE SYSTEMS; ORGANIC-SOLVENTS; PHASE; EXTRACTION; PROTEINS; BEHAVIOR; LACCASE |
abstract |
Herein, thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recovery and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes. |
publisher |
ROYAL SOC CHEMISTRY |
issn |
1463-9262 |
year published |
2018 |
volume |
20 |
issue |
6 |
beginning page |
1218 |
ending page |
1223 |
digital object identifier (doi) |
10.1039/c7gc03880a |
web of science category |
Chemistry, Multidisciplinary; Green & Sustainable Science & Technology |
subject category |
Chemistry; Science & Technology - Other Topics |
unique article identifier |
WOS:000428088400007
|