Preparation of Well-Dispersed Chitosan/Alginate Hollow Multilayered Microcapsules for Enhanced Cellular Internalization
authors Ribeiro, C; Borges, J; Costa, AMS; Gaspar, VM; Bermudez, VD; Mano, JF
nationality International
journal MOLECULES
author keywords chitosan; alginate; marine origin polysaccharides; biocompatible polymers; CaCO3 porous microparticles; layer-by-layer assembly; hollow multilayered microcapsules; non-aggregated microcapsules; cellular internalization
keywords POROUS CACO3 MICROPARTICLES; DRUG-DELIVERY; BIOMEDICAL APPLICATIONS; CALCIUM-CARBONATE; POLYELECTROLYTE MICROCAPSULES; PROTEIN ENCAPSULATION; POLYMER CAPSULES; PARTICLE UPTAKE; CELLS; SHELLS
abstract Hollow multilayered capsules have shown massive potential for being used in the biomedical and biotechnology fields, in applications such as cellular internalization, intracellular trafficking, drug delivery, or tissue engineering. In particular, hollow microcapsules, developed by resorting to porous calcium carbonate sacrificial templates, natural-origin building blocks and the prominent Layer-by-Layer (LbL) technology, have attracted increasing attention owing to their key features. However, these microcapsules revealed a great tendency to aggregate, which represents a major hurdle when aiming for cellular internalization and intracellular therapeutics delivery. Herein, we report the preparation of well-dispersed polysaccharide-based hollow multilayered microcapsules by combining the LbL technique with an optimized purification process. Cationic chitosan (CHT) and anionic alginate (ALG) were chosen as the marine origin polysaccharides due to their biocompatibility and structural similarity to the extracellular matrices of living tissues. Moreover, the inexpensive and highly versatile LbL technology was used to fabricate core-shell microparticles and hollow multilayered microcapsules, with precise control over their composition and physicochemical properties, by repeating the alternate deposition of both materials. The microcapsules' synthesis procedure was optimized to extensively reduce their natural aggregation tendency, as shown by the morphological analysis monitored by advanced microscopy techniques. The well-dispersed microcapsules showed an enhanced uptake by fibroblasts, opening new perspectives for cellular internalization.
publisher MDPI
issn 1420-3049
year published 2018
volume 23
issue 3
digital object identifier (doi) 10.3390/molecules23030625
web of science category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000428514100116
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journal analysis (jcr 2017):
journal impact factor 3.098
5 year journal impact factor 3.268
category normalized journal impact factor percentile 57.994
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