Investigating the effect of fibulin-1 on the differentiation of human nasal inferior turbinate-derived mesenchymal stem cells into osteoblasts
authors Pham, LBH; Yoo, YR; Park, SH; Back, SA; Kim, SW; Bjorge, I; Mano, J; Jang, JH
nationality International
journal JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
author keywords extracellular matrix proteins; fibulin-1; hTMSCs; osteoblast; osteogenic differentiation
keywords POROUS SILK FIBROIN; CIRCULATORY-SYSTEM; ARTERIAL TREES; FRACTAL MODELS; IN-VIVO; ANGIOGENESIS; VASCULARIZATION; PRINCIPLE; PATTERNS; SCAFFOLD
abstract Many extracellular matrix proteins have positive influences on the adhesion, proliferation, and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p<0.001) and proliferation of hTMSCs (p<0.05). The ALP and mineralization activities of cells were dramatically increased (p<0.01) after 9 and 12 days of FBLN1 treatment, respectively. This indicated the ability of FBLN1 to induce hTMSCs to differentiate into osteoblasts. Furthermore, increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration. (C) 2017 Wiley Periodicals, Inc.
publisher WILEY
issn 1549-3296
year published 2017
volume 105
issue 8
beginning page 2291
ending page 2298
digital object identifier (doi) 10.1002/jbm.a.36095
web of science category Engineering, Biomedical; Materials Science, Biomaterials
subject category Engineering; Materials Science
unique article identifier WOS:000404370600018
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journal analysis (jcr 2017):
journal impact factor 3.231
5 year journal impact factor 3.329
category normalized journal impact factor percentile 64.656
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