Multifunctional laminarin microparticles for cell adhesion and expansion
authors Martins, CR; Custodio, CA; Mano, JF
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Microfluidic; Microcarrier; Microgels; Platelet lysates; Methacrylated laminarin; Injectable scaffolds
keywords FETAL BOVINE SERUM; PLATELET LYSATE; MICROFLUIDIC FABRICATION; HYDROGEL PARTICLES; ALGINATE MICROGELS; TISSUE CONSTRUCTS; GROWTH-FACTORS; IN-VIVO; MICROSPHERES; DELIVERY
abstract Microfabrication technologies have been widely explored to produce microgels that can be assembled in functional constructs for tissue engineering and regenerative medicine applications. Here, we propose microfluidics coupled to a source of UV light to produce multifunctional methacrylated laminarin microparticles with narrow distribution of sizes using photopolyrnerization. The multifunctional microparticles were loaded with platelet lysates and further conjugated with an adhesive peptide. The adhesive peptides dictated cell adhesiveness to the laminarin microparticles, the incorporation of platelet lysates have resulted in improved cell expansion compared to clear microparticles. Overall, our findings demonstrate that multifunctional methacrylated laminarin microparticles provide an effective support for cell attachment and expansion. Moreover, expanded cells provide the link for microparticles aggregation resulting in robust 3D structures. This suggest the potential for using the methacrylated laminarin microplatforms capable to be assembled by the action of cells to rapidly produce large tissue engineered constructs.
publisher ELSEVIER SCI LTD
issn 0144-8617
year published 2018
volume 202
beginning page 91
ending page 98
digital object identifier (doi) 10.1016/j.carbpol.2018.08.029
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000446027100011
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 5.158
5 year journal impact factor 5.326
category normalized journal impact factor percentile 93.599
dimensions (citation analysis):
altmetrics (social interaction):



 


Apoio

1suponsers_list_ciceco.jpg