Acetonitrile as adjuvant to tune polyethylene glycol + K3PO4 aqueous two-phase systems and its effect on phenolic compounds partition
authors do Rosario, RLSD; Souza, RL; Farias, FO; Mafra, MR; Soares, CMF; Passos, H; Coutinho, JAP; Lima, AS
nationality International
journal SEPARATION AND PURIFICATION TECHNOLOGY
author keywords Liquid-liquid systems; Organic solvents; Kamlet-Taft parameters; Extraction; Phenolic compounds
keywords AQUEOUS 2-PHASE SYSTEMS; SOLVATOCHROMIC COMPARISON METHOD; PHASE-SEPARATION; BIPHASIC SYSTEMS; SUGARING-OUT; EXTRACTION; PROTEIN; SCALE; WATER; BEHAVIOR
abstract Aqueous two-phase systems (ATPS) have been proposed as platforms for the purification of biomolecules, and the application of adjuvants to tune the properties of ATPS phases and their ability to extract and separate biological products investigated. This work addresses, for the first time, the use of acetonitrile (ACN) as adjuvant in ATPS composed of polyethylene glycol (PEG) of different molecular weights (from 400 to 20,000 g.mol(-1)) and potassium phosphate. The effect of ACN concentration (at 0.25, 2.5, 5 and 7.5 wt%) in the liquid-liquid equilibrium is here studied by measuring the phase diagrams, the composition of the phases in equilibrium and their Kamlet-Taft parameters. The results obtained demonstrate that the ACN presence increases the biphasic region of PEG-K3PO4 ATPS and its distribution between the coexisting phases is dependent on its concentration and ATPS composition. The difference in the dipolarity/polarizability of the coexisting phases is significantly affected by the presence of ACN in the ternary mixture. The partition of two phenolic compounds - protocatechuic acid and gallic acid - was studied, showing that ACN improves the partition of these molecules to the salt-rich phase, and that the system selectivity can be significantly improved by changing the concentration of the phase forming compounds.
publisher ELSEVIER SCIENCE BV
issn 1383-5866
year published 2019
volume 223
beginning page 41
ending page 48
digital object identifier (doi) 10.1016/j.seppur.2019.04.062
web of science category Engineering, Chemical
subject category Engineering
unique article identifier WOS:000472697400006
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