Mesoporous Metal-Organic Frameworks as Effective Nucleating Agents in Protein Crystallography
authors Leite, JP; Rodrigues, D; Ferreira, S; Figueira, F; Paz, FAA; Gales, L
nationality International
journal CRYSTAL GROWTH & DESIGN
keywords HETEROGENEOUS NUCLEATION; ENZYME IMMOBILIZATION; CRYSTALS; RELEASE; CAGES
abstract Mesoporous metal-organic frameworks (mesoMOFs) have gained some attention as protein immobilization agents. Herein, and for the first time, three model proteins (lysozyme, trypsin, and albumin) were used to show that crystallization is effectively induced when the protein is immobilized inside the mesoMOF, with this occurring for lysozyme, while trypsin and albumin were size-excluded. It is shown that heterogeneous nucleation is truly remarkable in the case of lysozyme with a strong adhesion to the mesoMOF surface. These findings suggest that the ability to fix the target protein with a molecular-scale periodicity constitutes a significant advantage of mesoMOFs over other nucleating agents.
publisher AMER CHEMICAL SOC
issn 1528-7483
year published 2019
volume 19
issue 3
beginning page 1610
ending page 1615
digital object identifier (doi) 10.1021/acs.cgd.8b01444
web of science category Chemistry, Multidisciplinary; Crystallography; Materials Science, Multidisciplinary
subject category Chemistry; Crystallography; Materials Science
unique article identifier WOS:000460996600020
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 3.972
5 year journal impact factor 3.880
category normalized journal impact factor percentile 77.120
dimensions (citation analysis):
altmetrics (social interaction):



 


Apoio

1suponsers_list_ciceco.jpg