Biosynthesized composites of Au-Ag nanoparticles using Trapa peel extract induced ROS-mediated p53 independent apoptosis in cancer cells
authors Ahmad, N; Sharma, AK; Sharma, S; Khan, I; Sharma, DK; Shamsi, A; Kumar, TRS; Seervi, M
nationality International
journal DRUG AND CHEMICAL TOXICOLOGY
author keywords Trapa; Au-Ag nanoparticles; green-synthesis; bio-waste; apoptosis; p53
keywords GOLD NANOPARTICLES; PHENOLIC-COMPOUNDS; RAPID SYNTHESIS; ANTIOXIDANT; GREEN
abstract The current study highlights rapid, sustainable, and cost-effective biosynthesis of silver (Ag), gold (Au) nanoparticles (NPs), and bimetallic Au-AgNPs composites using bio-waste extract of Trapa natans. Growth of the NPs was monitored spectrophotometrically and peak was observed at similar to 525 nm, similar to 450 nm, and similar to 495 nm corresponding to Plasmon absorbance of AuNPs, AgNPs, and Au-AgNPs, respectively. Transmission electron microscopy (TEM) revealed the size of AgNPs (similar to 15 nm), AuNPs (similar to 25 nm), and Au-AgNPs (similar to 26-90 nm). Synthesized NPs follow the Gaussian bell curve and its crystalline nature was identified by X-ray diffraction (XRD). Furthermore, Au-AgNPs induced cytotoxicity in various cancer cells (HCT116, MDA-MB-231, and HeLa) effectively at 200 mu g/mL. Au-AgNPs-exposed cancer cells exhibited apoptotic features such as nuclear condensation, mitochondrial membrane potential loss, and cleavage of casp-3 and poly (ADP-ribose) polymerase-1 (PARP). Au-AgNPs exposure enhanced reactive oxygen species (ROS) and upon inhibition of ROS, apoptosis was reduced effectively. NPs treatment killed HCT116 WT and p53 knockout cells without any significant difference. Mechanistically, Au-AgNPs derived with Trapa peel extract significantly enhance ROS which trigger p53-independent apoptosis in various cancer cells effectively. Our study explores the use of bio-waste for the green synthesis of NPs, which can be attractive candidates for cancer therapy.
publisher TAYLOR & FRANCIS LTD
issn 0148-0545
year published 2019
volume 42
issue 1
beginning page 43
ending page 53
digital object identifier (doi) 10.1080/01480545.2018.1463241
web of science category Chemistry, Multidisciplinary; Pharmacology & Pharmacy; Toxicology
subject category Chemistry; Pharmacology & Pharmacy; Toxicology
unique article identifier WOS:000458887100005
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 1.531
5 year journal impact factor 1.475
category normalized journal impact factor percentile 26.097
dimensions (citation analysis):
altmetrics (social interaction):



 


Apoio

1suponsers_list_ciceco.jpg