|
authors |
Ferro, MD; Santos, SAO; Silvestre, AJD; Duarte, MF |
|
nationality |
International |
|
journal |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
|
author keywords |
extra virgin olive oil; hydrophilic phenolic compounds; chromatographic resolution; Kinetex biphenyl core-shell column; method validation |
|
keywords |
REVERSED-PHASE HPLC; POLYPHENOLS; OLEUROPEIN; 3,4-DIHYDROXYPHENYLGLYCOL; ANTIOXIDANTS; PERFORMANCE; HYDROLYSIS; TYROSOL |
|
abstract |
Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C-18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (n(c)), higher selectivity () (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (R-s) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs. |
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publisher |
MDPI |
|
isbn |
1422-0067 |
|
year published |
2019 |
|
volume |
20 |
|
issue |
1 |
|
digital object identifier (doi) |
10.3390/ijms20010201 |
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web of science category |
Biochemistry & Molecular Biology; Chemistry, Multidisciplinary |
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subject category |
Biochemistry & Molecular Biology; Chemistry |
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unique article identifier |
WOS:000459747700201
|
