NMR metabolomics to study the metabolic response of human osteoblasts to non-poled and poled poly (L-lactic) acid
authors Ara?jo, R; Carneiro, TJ; Marinho, P; da Costa, MM; Roque, A; Silva, OABDE; Fernandes, MH; Vilarinho, PM; Gil, AM
nationality International
journal MAGNETIC RESONANCE IN CHEMISTRY
author keywords H-1; metabolomics; NMR; osteoblasts; piezoelectricity; poling; poly-L-lactic acid (PLLA); tissue regeneration
keywords CELLS; STEM; NANOFIBERS; SCAFFOLDS; OXYGEN; TOOL; DIFFERENTIATION; CONSUMPTION
abstract Untargeted nuclear magnetic resonance (NMR) metabolomics was employed, for the first time to our knowledge, to characterize the metabolome of human osteoblast (HOb) cells and extracts in the presence of non-poled or negatively poled poly-L-lactic acid (PLLA). The metabolic response of these cells to this polymer, extensively used in bone regeneration strategies, may potentially translate into useful markers indicative of in vivo biomaterial performance. We present preliminary results of multivariate and univariate analysis of NMR spectra, which have shown the complementarity of lysed cells and extracts in terms of information on cell metabolome, and unveil that, irrespective of poling state, PLLA-grown cells seem to experience enhanced oxidative stress and activated energy metabolism, at the cost of storage lipids and glucose. Possible changes in protein and nucleic acid metabolisms were also suggested, as well as enhanced membrane biosynthesis. Therefore, the presence of PLLA seems to trigger cell catabolism and anti-oxidative protective mechanisms in HOb cells, while directing them towards cellular growth. This was not sufficient, however, to lead to a visible cell proliferation enhancement in the presence of PLLA, although a qualitative tendency for negatively poled PLLA to be more effective in sustaining cell growth than non-poled PLLA was suggested. These preliminary results indicate the potential of NMR metabolomics in enlightening cell metabolism in response to biomaterials and their properties, justifying further studies of the fine effects of poled PLLA on these and other cells of significance in tissue regeneration strategies.
publisher WILEY
issn 0749-1581
year published 2019
volume 57
issue 11
beginning page 919
ending page 933
digital object identifier (doi) 10.1002/mrc.4883
web of science category Chemistry, Multidisciplinary; Chemistry, Physical; Spectroscopy
subject category Chemistry; Spectroscopy
unique article identifier WOS:000492751600004
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journal impact factor 1.776
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