Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography
authors Santos, JHPM; Ferreira, AM; Almeida, MR; Quinteiro, PSGN; Dias, ACRV; Coutinho, JAP; Freire, MG; Rangel-Yagui, CO; Ventura, SPM
nationality International
journal GREEN CHEMISTRY
keywords AQUEOUS 2-PHASE SYSTEMS; EXTRUSION COUNTERCURRENT CHROMATOGRAPHY; POLYETHYLENE-GLYCOL; L-ASPARAGINASE; CHEMISTRY; PHASE; FRACTIONATION; PURIFICATION; PROTEINS; RESIDUES
abstract Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (similar to 100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units.
publisher ROYAL SOC CHEMISTRY
issn 1463-9262
year published 2019
volume 21
issue 20
beginning page 5501
ending page 5506
digital object identifier (doi) 10.1039/c9gc01063g
web of science category Chemistry, Multidisciplinary; Green & Sustainable Science & Technology
subject category Chemistry; Science & Technology - Other Topics
unique article identifier WOS:000490291800024

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