Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio
authors Martin-Pastor, M; Ferreira, AS; Moppert, X; Nunes, C; Coimbra, MA; Reis, RL; Guezennec, J; Novoa-Carballal, R
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Exopolysaccharide; Hyaluronic-like; NMR spectroscopy; Physicochemical properties; Bacterial mats; Vibrio sp
keywords SEA HYDROTHERMAL BACTERIUM; MOLECULAR-WEIGHT; SODIUM HYALURONATE; MICROBIAL MATS; ACID; POLYSACCHARIDE; VISCOSITY; ALGINATE; BEHAVIOR
abstract MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit -> 4)-beta-D-GlcpA-(1 -> 4)-alpha-D-GalpNAc-(1 -> 3)-beta-D-GlcpNAc-(1 -> 4)-beta-D-GlcpA-(1 -> containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (R-g) and intrinsic viscosity, [eta], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 +/- 4 kDa (PDI, 1.42 +/- 0.01), 6.7 +/- 0.3 dl/g and 56 +/- 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1 -> 3 and 1 -> 4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.
publisher ELSEVIER SCI LTD
issn 0144-8617
year published 2019
volume 222
digital object identifier (doi) 10.1016/j.carbpol.2019.114999
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000475425500058
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journal impact factor 5.158
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