Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides
authors Pandeirada, CO; Maricato, E; Ferreira, SS; Correia, VG; Pinheiro, BA; Evtuguin, DV; Palma, AS; Correia, A; Vilanova, M; Coimbra, MA; Nunes, C
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Mixed-linked glucans; Sulphated polysaccharides; Mannans; Rhamnans; Carbohydrate microarrays; Immunity
keywords MONOCLONAL-ANTIBODY; CHEMICAL-STRUCTURE; MARINE MICROALGAE; AMINO-ACID; IMMUNOMODULATION; POLYMERS; LOCATION; OCEANICA; NITROGEN; GLUCANS
abstract The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (beta 1 -> 3, beta 1 -> 4)-glucans, (alpha 1 -> 3)-, (alpha 1 -> 4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.
publisher ELSEVIER SCI LTD
issn 0144-8617
isbn 1879-1344
year published 2019
volume 222
digital object identifier (doi) 10.1016/j.carbpol.2019.06.001
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000475425500054
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  impact metrics
journal analysis (jcr 2019):
journal impact factor 7.182
5 year journal impact factor 6.89
category normalized journal impact factor percentile 96.638
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