Supramolecular Presentation of Hyaluronan onto Model Surfaces for Studying the Behavior of Cancer Stem Cells
authors Pang, XQ; O'Malley, C; Borges, J; Rahman, MM; Collis, DWP; Mano, JF; Mackenzie, IC; Azevedo, HS
nationality International
journal ADVANCED BIOSYSTEMS
author keywords cancer stem cells; hyaluronan; model surfaces; peptides; self-assembled monolayers
keywords PROTEIN ADSORPTION; HYDROPHOBIC SURFACES; INTEGRIN BINDING; ACID; EXPRESSION; HEAD; CHEMISTRY; OLIGOSACCHARIDES; POLYSACCHARIDE; SPECIFICITY
abstract The supramolecular presentation of extracellular matrix components on surfaces provides a platform for the investigation and control of cell behavior. Hyaluronan (HA) is one of the main components of the extracellular environment and has been shown to play an important role in different cancers and their progression. However, current methods of HA immobilization often require its chemical modification. Herein, a peptide-based self-assembled monolayer (SAM) is used as an anchor to immobilize unmodified HA on a bare gold surface, as demonstrated by the quartz crystal microbalance with dissipation monitoring. Peptide-HA surfaces show increased roughness and greater hydrophobicity when compared to poly-D-lysine/HA surfaces, as measured by atomic force microscopy and water contact angle, respectively. Additionally, the peptide SAM can be micro-contact printed and used to restrict the presentation of HA to specific regions, thereby creating HA patterned surfaces to examine cell behavior. When used for cell culture, these surfaces result in altered adhesion and migration of LUC4 head and neck squamous cell carcinoma cells. These biomimetic surfaces can provide insights into the role of HA in cancer and other diseases and be used as a platform for the development of cell sorting devices.
publisher WILEY-V C H VERLAG GMBH
year published 2019
volume 3
issue 10
digital object identifier (doi) 10.1002/adbi.201900017
web of science category Materials Science, Biomaterials
subject category Materials Science
unique article identifier WOS:000482393900001
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