Contribution of non-enzymatic transglycosylation reactions to the honey oligosaccharides origin and diversity
authors Silva, SR; Moreira, ASP; Domingues, MDM; Evtuguin, DV; Coelho, E; Coimbra, MA
nationality International
journal PURE AND APPLIED CHEMISTRY
author keywords ESI-MS; honey ripening; ICS-29; inulobiose; 1-kestose; maltose; methylation analysis; oligosaccharides formation; orbitrap-mass spectrometry; reversion reactions
keywords IDENTIFICATION; TRISACCHARIDES; ACIDITY; STORAGE; SUGARS
abstract Non-enzymatic transglycosylation reactions are known to occur under high sugar concentrations, high temperatures, low moisture environments and acidic conditions. Although honey is not a thermally processed food, its high sugars concentration under an acidic and low moisture environment for prolonged periods of time may also promote these reactions. To test this hypothesis six model solutions containing combinations of sucrose and glucose or fructose, prepared with water and diluted citric acid at pH 4.0 and 2.0, were incubated at 35 degrees C during up to 5 months, similar to the honey under hive conditions. Electrospray ionization mass spectrometry (ESI-MS) allowed to observe polymerization products soon after their incubation. After 5 months, a degree of polymerization of 6 was detected, similarly to the honey samples used for comparison. Maltose, isomaltose, inulobiose, sophorose, gentiobiose, 1-kestose and panose were detected in both model solutions and honey samples, showing that non-enzymatic transglycosylation reactions also contribute to oligosaccharides origin and diversity in honey.
publisher WALTER DE GRUYTER GMBH
issn 0033-4545
isbn 1365-3075
year published 2019
volume 91
issue 7
beginning page 1231
ending page 1242
digital object identifier (doi) 10.1515/pac-2019-0209
web of science category Chemistry, Multidisciplinary
subject category Chemistry
unique article identifier WOS:000475296300012
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 1.919
5 year journal impact factor 4.415
category normalized journal impact factor percentile 38.701
dimensions (citation analysis):
altmetrics (social interaction):



 


Apoio

1suponsers_list_ciceco.jpg