Gd- and Eu-Loaded Iron Oxide@Silica Core-Shell Nanocomposites as Trimodal Contrast Agents for Magnetic Resonance Imaging and Optical Imaging
authors Pinho, SLC; Sereno, J; Abrunhosa, AJ; Delville, MH; Rocha, J; Carlos, LD; Geraldes, CFGC
nationality International
journal INORGANIC CHEMISTRY
keywords CELLULAR UPTAKE; NANOPARTICLES; MRI; T-1; SIZE; NANOCRYSTALS; LUMINESCENCE; NANOCUBES; DESIGN; GROWTH
abstract Superparamagnetic maghemite core-porous silica shell nanoparticles, gamma-Fe2O3@SiO2 (FS), with 50 nm diameter and a 10 nm core, impregnated with paramagnetic complexes b-Ln ([Ln(btfa)(3)(H2O)(2)]) (where btfa = 4,4,4-trifluoro-L-phenyl-1,3-butanedione and Ln = Gd, Eu, and Gd/Eu), performing as promising trimodal T-1-T-2 MRI and optical imaging contrast agents, are reported. These nanosystems exhibit a high dispersion stability in water and no observable cytotoxic effects, witnessed by intracellular ATP levels. The structure and superparamagnetic properties of the maghemite core nanocrystals are preserved upon imbedding the b-Ln complexes in the shell. Hela cells efficiently and swiftly internalize the NPs into the cytosol, with no observable cytotoxicity below a concentration of 62.5 mu g mL(-1). These nanosystems perform better than the free b-Gd complex as T-1 (positive) contrast agents in cellular pellets, while their performance as T-2 (negative) contrast agents is similar to the FS. Embedding of the b-Eu complex in the silica pores endows the nanoparticles with strong luminescence properties. The impregnation of gadolinium and europium complexes in a 1:1 ratio afforded a trimodal nanoplatform performing as a luminescent probe and a double T-1 and T-2 MRI contrast agent even more efficient than b-Gd used on its own, as observed in cell-labeled imaging experiments and MRI cell pellets.
publisher AMER CHEMICAL SOC
issn 0020-1669
isbn 1520-510X
year published 2019
volume 58
issue 24
beginning page 16618
ending page 16628
digital object identifier (doi) 10.1021/acs.inorgchem.9b02655
web of science category Chemistry, Inorganic & Nuclear
subject category Chemistry
unique article identifier WOS:000503330800032
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journal analysis (jcr 2019):
journal impact factor 4.825
5 year journal impact factor 4.501
category normalized journal impact factor percentile 92.222
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