Aqueous solutions of deep eutectic systems as reaction media for the saccharification and fermentation of hardwood xylan into xylitol
authors Romani, A; Morais, ES; Soares, PO; Freire, MG; Freire, CSR; Silvestre, AJD; Domingues, L
nationality International
journal BIORESOURCE TECHNOLOGY
author keywords Xylitol; Cholinium chloride; Urea; Xylan; Simultaneous saccharification and fermentation
keywords SACCHAROMYCES-CEREVISIAE; LIGNOCELLULOSIC BIOMASS; BIOETHANOL PRODUCTION; ENZYMATIC-HYDROLYSIS; SOLVENTS; PRETREATMENT; LIGNIN; XYLOSE; VALORIZATION; CONVERSION
abstract The aim of this study was to evaluate the effect of aqueous solutions of deep eutectic solvent, Cholinium Chloride:Urea ([Ch]Cl:U) at 50 wt% and 20 wt%, using different molar ratios (1:1, 2:1 and 1:2) on the enzymatic hydrolysis of xylan for xylose production and its subsequent bioconversion into xylitol using a recombinant yeast strain. The lowest xylan conversion into xylose (45%) was obtained using 1:2 [Ch]Cl:U molar ratio. On the other hand, the 1:1 [Ch]Cl:U molar ratio, at 20 wt% in water, improved this conversion, achieving the highest xylose yield (81.4%). The xylitol production was then optimized with [Ch]Cl:U (1:1) at 20 wt% by simultaneous saccharification and fermentation process, attaining 23.67 g/L, corresponding to 66.04% of xylitol yield. This study reveals the possibility of using xylan solubilized in DES aqueous solutions directly for xylitol production, thus assembling a one-step process.
publisher ELSEVIER SCI LTD
issn 0960-8524
isbn 1873-2976
year published 2020
volume 311
digital object identifier (doi) 10.1016/j.biortech.2020.123524
web of science category Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
subject category Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels
unique article identifier WOS:000539210000003

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