Continuous flow reactor based with an immobilized biocatalyst for the continuous enzymatic transesterification of crude coconut oil
authors Santana, JL; Oliveira, JM; Nascimento, JS; Mattedi, S; Krause, LC; Freitas, LS; Cavalcanti, EB; Pereira, MM; Lima, AS; Soares, CMF
nationality International
journal BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
author keywords transesterification; packed bed reactor; molecular docking; protic ionic liquid
keywords PACKED-BED REACTOR; BIODIESEL PRODUCTION; IONIC LIQUIDS; ETHYL-ESTERS; LIPASE IMMOBILIZATION; BURKHOLDERIA-CEPACIA; PALM OIL; CONFORMATION; GLYCEROL; SIMULATION
abstract Here, we have assessed the use of one packed bed or two packed bed reactors in series in which Burkholderia cepacia lipase (BCL) was immobilized on protic ionic liquid (PIL)-modified silica and used as a biocatalyst for the transesterification of crude coconut oil. Reaction parameters including volumetric flow, temperature, and molar ratio were evaluated. The conversion of transesterification reaction products (ethyl esters) was determined using gas chromatography and the quantities of intermediate products (diglyceride and monoglyceride [MG]) were assessed using high-performance liquid chromatography. Packed bed reactors in series produced ethyl esters with the greatest efficiency, achieving 65.27% conversion after 96 H at a volumetric flow rate of 0.50 mL Min(-1) at 40 degrees C and a 1:9 molar ratio of oil to ethanol. Further, within the first 24 H of the reaction, increased MG (54.5%) production was observed. Molecular docking analyses were performed to evaluate the catalytic step of coconut oil transesterification in the presence of BCL. Molecular docking analysis showed that triglycerides have a higher affinity energy (-5.7 kcal mol(-1)) than the smallest MG (-6.0 kcal mol(-1)), therefore, BCL catalyzes the conversion of triglycerides rather than MG, which is consistent with experimental results.
publisher WILEY
issn 0885-4513
isbn 1470-8744
year published 2020
volume 67
issue 3
beginning page 404
ending page 413
digital object identifier (doi) 10.1002/bab.1885
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000510030700001
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journal impact factor 1.638
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