Mechanochemical Patternable ECM-Mimetic Hydrogels for Programmed Cell Orientation
authors Lavrador, P; Gaspar, VM; Mano, JF
nationality International
journal ADVANCED HEALTHCARE MATERIALS
author keywords dynamic covalent bonds; hydrogels; mechanically imprintable patterns; nanotopography; Schiff base
keywords PERIODATE-OXIDATION; STRESS-RELAXATION; GELATIN HYDROGELS; LYSYL OXIDASE; STEM-CELLS; DIFFERENTIATION; MATRIX; POLYSACCHARIDES; ADHESION; CULTURE
abstract Native human tissues are supported by a viscoelastic extracellular matrix (ECM) that can adapt its intricate network to dynamic mechanical stimuli. To recapitulate the unique ECM biofunctionality, hydrogel design is shifting from typical covalent crosslinks toward covalently adaptable networks. To pursue such properties, herein hybrid polysaccharide-polypeptide networks are designed based on dynamic covalent assembly inspired by natural ECM crosslinking processes. This is achieved through the synthesis of an amine-reactive oxidized-laminarin biopolymer that can readily crosslink with gelatin (oxLAM-Gelatin) and simultaneously allow cell encapsulation. Interestingly, the rational design of oxLAM-Gelatin hydrogels with varying aldehyde-to-amine ratios enables a refined control over crosslinking kinetics, viscoelastic properties, and degradability profile. The mechanochemical features of these hydrogels post-crosslinking offer an alternative route for imprinting any intended nano- or microtopography in ECM-mimetic matrices bearing inherent cell-adhesive motifs. Different patterns are easily paved in oxLAM-Gelatin under physiological conditions and complex topographical configurations are retained along time. Human adipose-derived mesenchymal stem cells contacting mechanically sculpted oxLAM-Gelatin hydrogels sense the underlying surface nanotopography and align parallel to the anisotropic nanoridge/nanogroove intercalating array. These findings demonstrate that covalently adaptable features in ECM-mimetic networks can be leveraged to combine surface topography and cell-adhesive motifs as they appear in natural matrices.
publisher WILEY
issn 2192-2640
year published 2020
volume 9
issue 10
digital object identifier (doi) 10.1002/adhm.201901860
web of science category Engineering, Biomedical; Nanoscience & Nanotechnology; Materials Science, Biomaterials
subject category Engineering; Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000527636200001
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journal impact factor 7.367
5 year journal impact factor 6.964
category normalized journal impact factor percentile 87.158
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