|
authors |
Sequeira, RA; Sharma, M; Pereira, MM; Singh, N; Bhattacharya, S; Chudasama, NA; Prasad, K |
|
nationality |
International |
|
journal |
SEPARATION SCIENCE AND TECHNOLOGY |
|
author keywords |
Ionic liquid; epsilon-poly-L-lysine; polyamide; aqueous biphasic system; separation |
|
keywords |
2-PHASE SYSTEMS; PROTEIN SEPARATION; EXTRACTION; WATER |
|
abstract |
Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate epsilon-PL from culture broth. It was observed that the partition of epsilon-PL to the IL-rich phase was achieved, and with pure epsilon-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of epsilon-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of epsilon-PL from the culture broth. |
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publisher |
TAYLOR & FRANCIS INC |
|
issn |
0149-6395 |
|
year published |
2020 |
|
digital object identifier (doi) |
10.1080/01496395.2020.1728323 |
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web of science category |
Chemistry, Multidisciplinary; Engineering, Chemical |
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subject category |
Chemistry; Engineering |
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unique article identifier |
WOS:000514054200001
|
