Self-Assembled Bioactive Colloidal Gels as Injectable Multiparticle Shedding Platforms
authors Freitas, B; Lavrador, P; Almeida, RJ; Gaspar, VM; Mano, JF
nationality International
journal ACS APPLIED MATERIALS & INTERFACES
author keywords biomaterials; bottom-up self-assembly; colloidal gels; dynamic network; nanoparticles Unitary
keywords DRUG-DELIVERY SYSTEMS; HYDROGEL
abstract Self-assembled colloidal gels are highly versatile 3D nanocluster platforms with potential to overcome the rapid clearing issues associated with standard free nanotherapeutics administration. However, the development of nanoassembled colloidal gels exhibiting autonomous multiparticle release from the bulk particle network remains elusive. Herein, we generated multiparticle colloidal gels from two nanosized building blocks: cationic poly(Dplactide-co-glycolide)-polyethylenimine (PLGA-PEI) nanoparticles and anionic zein-hyaluronan (HA) nanogels that assemble into macrosized 3D constructs via attractive electrostatic forces. The resulting colloidal gels exhibited high stability in complex culture medium as well as fitto-shape moldable properties and injectability. Moreover, nanoassembled colloidal gels encapsulated bioactive quercetin flavonoids with high loading efficacy and presented remarkable antiinflammatory activities, reducing key proinflammatory biomarkers in inflammation-activated macrophages. More importantly, because of their rationally selected building blocks zein-HA/PLGA-PEI, self-assembled colloidal platforms displayed autonomous multiparticle shedding. Both positive and negative particles released from the colloidal system were efficiently internalized by macrophages along time as evidenced by quantitative particle uptake analysis. Overall, the generated nanostructured gels represent an implantable versatile platform for focalized multiparticle delivery. In addition, the possibility to combine a higher number of particle species with different properties or stimuli-responsiveness enables the manufacturing of combinatorial nanostructured gels for numerous biomedical applications.
publisher AMER CHEMICAL SOC
issn 1944-8244
year published 2020
volume 12
issue 28
beginning page 31282
ending page 31291
digital object identifier (doi) 10.1021/acsami.0c09270
web of science category Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
subject category Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000551488400021

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