Separation and purification of biomacromolecules based on microfluidics
authors Vicente, FA; Plazl, I; Ventura, SPM; Znidarsic-Plazl, P
nationality International
journal GREEN CHEMISTRY
keywords AQUEOUS 2-PHASE EXTRACTION; PROTEIN CRYSTALLIZATION CONDITIONS; SELF-INTERACTION CHROMATOGRAPHY; LIQUID-LIQUID-EXTRACTION; SOLID-PHASE EXTRACTION; CAPILLARY-ELECTROPHORESIS; IONIC LIQUIDS; HUMAN SERUM; PROCESS INTENSIFICATION; SERIAL CRYSTALLOGRAPHY
abstract Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidicsbased separation and purification of proteins and nucleic acids with the focus on liquid-liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.
publisher ROYAL SOC CHEMISTRY
issn 1463-9262
isbn 1463-9270
year published 2020
volume 22
issue 14
beginning page 4391
ending page 4410
digital object identifier (doi) 10.1039/c9gc04362d
web of science category Chemistry, Multidisciplinary; Green & Sustainable Science & Technology
subject category Chemistry; Science & Technology - Other Topics
unique article identifier WOS:000550567200031
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journal analysis (jcr 2019):
journal impact factor 9.48
5 year journal impact factor 9.707
category normalized journal impact factor percentile 92.662
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