Towards the differential diagnosis of prostate cancer by the pre-treatment of human urine using ionic liquids
authors Pereira, MM; Calixto, JD; Sousa, ACA; Pereira, BJ; Lima, AS; Coutinho, JAP; Freire, MG
nationality International
journal SCIENTIFIC REPORTS
keywords AQUEOUS BIPHASIC SYSTEMS; GOODS BUFFERS; ANTIGEN; SERUM; SALTS; ASSAY; EXTRACTION; WATER; PSA
abstract Prostate specific antigen (PSA) is the most widely used clinical biomarker for the diagnosis and monitoring of prostate cancer. Most available techniques for PSA quantification in human fluids require extensive sample processing and expensive immunoassays that are often unavailable in developing countries. The quantification of PSA in serum is the most common practice; however, PSA is also present in human urine, although less used in diagnosis. Herein we demonstrate the use of ionic-liquid-based aqueous biphasic systems (IL-based ABS) as effective pre-treatment strategies of human urine, allowing the PSA detection and quantification by more expedite equipment in a non-invasive matrix. If properly designed, IL-based ABS afford the simultaneous extraction and concentration of PSA (at least up to 250-fold) in the IL-rich phase. The best ABS not only allow to concentrate PSA but also other forms of PSA, which can be additionally quantified, paving the way to their use in differential prostate cancer diagnosis.
publisher NATURE RESEARCH
issn 2045-2322
year published 2020
volume 10
issue 1
digital object identifier (doi) 10.1038/s41598-020-71925-8
web of science category Multidisciplinary Sciences
subject category Science & Technology - Other Topics
unique article identifier WOS:000571915100052
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journal analysis (jcr 2019):
journal impact factor 3.998
5 year journal impact factor 4.576
category normalized journal impact factor percentile 76.761
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