Protein Cohabitation: Improving the Photochemical Stability of R-Phycoerythrin in the Solid State
authors Bharmoria, P; Correia, SFH; Martins, M; Hernandez-Rodriguez, MA; Ventura, SPM; Ferreira, RAS; Carlos, LD; Coutinho, JAP
nationality International
journal JOURNAL OF PHYSICAL CHEMISTRY LETTERS
keywords SINGLET OXYGEN; PHYCOBILIPROTEINS; COOPERATIVITY; PHYCOCYANIN; CELLS
abstract The poor photochemical stability of R-phycoerythrin (R-PE) has been a bottleneck for its broad-spectrum applications. Inspired by nature, we studied a sustainable strategy of protein cohabitation to enhance R-PE stability by embedding it in a solid matrix of gelatin. Both pure R-PE and fresh phycobiliprotein (PBP) extracts recovered from Gracilaria gracilis were studied. The incorporation of R-PE in the gelatin-based films (gelatin-RPE and gelatin-PBPs) has improved its photochemical stability for at least 8 months, the longest time period reported so far. These results were evidenced by not only absorption but also emission quantum yield measurements (Phi). Moreover, the photostability of gelatin-RPE films upon continuous excitation with an AM1.5G solar simulator was tested and found to remain stable for 23 h after initial decreasing up to 250 min. In the end, another approach was established to allow 100% photostability for a 3 h exposure to an AM1.5G solar simulator by doping the gelatin-based film including R-Phycoerythrin with n-propyl gallate stabilized with Tween 80, allowing their use as naturally based optically active centers in photovoltaic applications.
publisher AMER CHEMICAL SOC
issn 1948-7185
year published 2020
volume 11
issue 15
beginning page 6249
ending page 6255
digital object identifier (doi) 10.1021/acs.jpclett.0c01491
web of science category Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Atomic, Molecular & Chemical
subject category Chemistry; Science & Technology - Other Topics; Materials Science; Physics
unique article identifier WOS:000562064500063
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journal impact factor 6.71
5 year journal impact factor 7.811
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